High efficiency Rabbit anti GST-Tag pAb

Cat.No. BT8005V Size: 10ug Datasheet Request

Product Information

Clonallity: Monoclonal

Applications: WB, IP, FC, ELISA

Source: Rabbit

Immunogen: Recombinant Protein

Concentration: 1mg/ml

Observed molecular weight: 26 kDa

Formulation

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol

Purification method:

Antigen affinity purification

Species specificity:

recombinant protein, human

Positive WB detected in:

Recombinant protein

Positive FC detected in:

HeLa cells

Synonyms:

Glutathione S Transferase mu,glutathione-S-transferase, Glutathione S Transferase, Glutathione S transferase Mu 1, GST 26, GST class mu 1, GST1, GSTM1, GSTM1 1, GSTM1a 1a, GSTM1b 1b, GTH4, GTM1, H B, HB subunit 4, MGC26563, MU 1, MU, SJ26 antigen, SjGST, GST-tag

Recommended Dilution:

WB : 1:1000-1:4000

Not yet tested in other applications

Storage:

PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Background

GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.

Western blot analysis of GST-tagged protein using GST-tag antibody at different dilution. Each lane was loaded with 5 ug protein.

Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.

Lysates/proteins: 25ug per lane.

Blocking buffer: 3% nonfat dry milk in TBST.

Shanghai Korain Biotech Co. was built in 2010. As an creator in reagents and tools for life science, Korainbio provide researchers with tools and scientific support including 30,000 antibodies, 1000+ proteins and 5000 ELISA kits. We aim to be a leading provider with world-class level for the researchers all over the world. Our products line covers a set of research areas including Immunology, Neuroscience, Cancer, Kinases, Phosphatases and Cell Biology.

Bioassay Technology Laboratory (BT Lab) as a leading brand of Korainbio focused on helping researchers to optimize life science work and serves as a provider of test and developing services, including elisa tests, WB test and antigen development. Founding in 2010, our strategic focus has been on the development of enabling technologies in the research, development, manufacture and marketing of innovative immuno products and services based on molecular technologies.

Korain’s reagents are supported by superior processional team and a quality management system that is certified for CE and ISO. Our product development program for use in a variety of applications including:

ELISA and immunohistochemistry

• Immunoprecipitation
• Immunohistochemistry
• Immunofluorescence microscopy
• Immunohistochemistry
• Quantitative Multiplexing

About BT Lab

As a leading brand of Korainbio, Bioassay Technology Laboratory (BT Lab) offers a variety of cost-effective ELISA kits and sets to measure cytokines, chemokines, and soluble biomarkers consistently and reliably. Sandwich kit and Competitive that provide the core reagents our selection of ELISA products meets the demand of ELISA beginners to experts alike at a very economical price.

Worldwide Distributors

ELISA Kit Categories

Sandwich Kit
Sandwich kits are fully validated and ready-to-use, containing 96- well strip plates pre-coated with capture antibody to detect sample antigen.

Competitive Kit
In a competitive ELISA assay, sample antigen and labeled antigen compete for capture antibody binding. The more target protein there is in the sample, the less labeled antigen will be captured and the weaker the signal.

Qualitative Kit
Qualitative results provide a simple positive or negative result for a sample. In quantitative ELISA, the optical density (OD) of the sample is compared to a standard curve, which is typically a serial dilution of a known-concentration solution of the target molecule. It is suitable for detecting small antigens.

Advantages of our Sandwich kit

Without Diluting Sample

• Save assay time
• Wide detection range
• Reducing non-specific interactions between the sample matrix proteins

Streptavidin-biotin system

• Significant non-specific binding can be prevented by strepvidin
• Reducing interference from residual biotin in sample
• A non-covalent interaction with high affinity

Washing Step

• Only 5 times for one step

Pre-Coated plate

What We Provide

• Product datasheet, product image, MSDS, publican references
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What We Aim to

• Optimize your R&D with our tools
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• Devoted ourselves to providing researchers with professional support

If you’re interested in working with us to expand the market, please feel free to contact us by: save@bt-laboratory.com